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iscripttm cdna synthesis kit  (Bio-Rad)


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    Structured Review

    Bio-Rad iscripttm cdna synthesis kit
    Iscripttm Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 48978 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+cdna+synthesis+kit/iScript+cDNA+Synthesis+Kit/pm42905847-445-16-20
    Average 99 stars, based on 48978 article reviews
    iscripttm cdna synthesis kit - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: Urinary KIM-1 Transcript Detectability in Workers Chronically Exposed to Arsenic and Antimony in an Industrial Area of Central Chile: A Cross-Sectional Pilot Study.
    Article Snippet: Background: Chronic arsenic (As) and antimony (Sb) exposure causes renal toxicity in experimental models, but early molecular indicators of tubular stress in humans remain uncharacterised.. Jo ur na l P re -p ro of Methods: We assessed 12 male workers from Puchuncaví, Chile.. Urinary and blood As and Sb were quantified by hydride-generation atomic fluorescence spectrometry (HG-AFS).

    Article Title: A Combination of Ursolic Acid and Oleanolic Acid Disrupts Bioenergetic Homeostasis in Prostate Cancer and Inhibits Cancer Growth by Cytotoxic Autophagy.
    Article Snippet: .. Total RNA from treated and control cells was isolated using Aurum Total RNA Mini Kit (Bio-Rad Laboratories, Hercules, CA, USA) and cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad Laboratories) as per manufacturer protocol. .. The qPCR was performed on Applied Biosystems QuantStudio 3 Real-Time PCR system using the Universal SYBR Green Fast qPCR mix (Bio-Rad Laboratories) as described before [57].

    Article Title: Diarylheptanoids oxyphyllacinol, yakuchinone A, and yakuchinone B attenuate 12-O-tetradecanoylphorbol-13-acetate-induced acute skin inflammation in mice
    Article Snippet: Diarylheptanoids from Alpinia oxyphylla are potential anti-inflammatory compounds, but existing evidence is limited and has largely focused on YAK-A and YAK-B, with little direct comparative evaluation of structurally related diarylheptanoids in inflammatory models.. Here, we compared the protective effects of oxyphyllacinol (OXY), yakuchinone A (YAK-A), and yakuchinone B (YAK-B) in a 12-O-tetradecanoylphorbol-13-acetate (TPA)induced mouse ear inflammation model. Topical pretreatment with OXY, YAK-A, or YAK-B significantly attenuated TPA-induced ear edema and tissue thickening, with YAK-B showing the greatest reduction in edema under the experimental conditions examined.. These compounds differentially regulated inflammatory cytokine expression, with OXY showing more consistent inhibition of IL-1β and IL-6 at the protein level.

    Article Title: Enhanced Prophylactic Nephroprotective Effects of Nanoliposomal Nootkatone Against Cisplatin-Induced Renal Injury: Associations with NRF2/HO-1, TLR4/NF-κB, Apoptosis-Related (Bax/Bcl-2/Caspase-3), and Ferroptosis-Related (GPX4/SLC7A11) Markers.
    Article Snippet: This study investigated the prophylactic nephroprotective effects of crude nootkatone (NKT) and its nanoliposomal formulation (NKT-LNPs) against cisplatin-induced renal injury in rats.. Sixty male Sprague–Dawley rats were randomly allocated into six groups: control, NKT, NKT-LNPs, cisplatin, cisplatin + NKT, and cisplatin + NKT-LNPs.. NKT or NKT-LNPs (10 mg/kg NKT equivalent) was administered orally as prophylactic treatment for 7 days, with a single cisplatin injection (7.5 mg/kg, i.p.) on day 7.

    Article Title: Arginine Supplementation for Juvenile Florida Pompano ( Trachinotus carolinus ) in Practical Plant‐Based Diets
    Article Snippet: .. Complementary DNA was synthesized using the iScript cDNA Synthesis Kit (Bio‐Rad Laboratories, Hercules, California, USA). ..

    cDNA Synthesis:

    Article Title: Urinary KIM-1 Transcript Detectability in Workers Chronically Exposed to Arsenic and Antimony in an Industrial Area of Central Chile: A Cross-Sectional Pilot Study.
    Article Snippet: Background: Chronic arsenic (As) and antimony (Sb) exposure causes renal toxicity in experimental models, but early molecular indicators of tubular stress in humans remain uncharacterised.. Jo ur na l P re -p ro of Methods: We assessed 12 male workers from Puchuncaví, Chile.. Urinary and blood As and Sb were quantified by hydride-generation atomic fluorescence spectrometry (HG-AFS).

    Article Title: A Combination of Ursolic Acid and Oleanolic Acid Disrupts Bioenergetic Homeostasis in Prostate Cancer and Inhibits Cancer Growth by Cytotoxic Autophagy.
    Article Snippet: .. Total RNA from treated and control cells was isolated using Aurum Total RNA Mini Kit (Bio-Rad Laboratories, Hercules, CA, USA) and cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad Laboratories) as per manufacturer protocol. .. The qPCR was performed on Applied Biosystems QuantStudio 3 Real-Time PCR system using the Universal SYBR Green Fast qPCR mix (Bio-Rad Laboratories) as described before [57].

    Article Title: Hesperidin Attenuates Vincristine‐Induced Nephrotoxicity via Nrf2/HO‐1 and PI3K/Akt/mTOR Signaling
    Article Snippet: The RNA concentrations were standardized across samples, and the RNA purity and quantity were assessed using a NanoDrop spectrophotometer (BioTek Instruments, EPOCH, USA). .. In accordance with the manufacturer's instructions, the iScript cDNA Synthesis Kit (Bio‐Rad, USA) was employed to generate complementary DNA (cDNA). .. The qRT‐PCR was conducted using a Rotor‐Gene Q system (Qiagen, Germany) and iTaq Universal SYBR Green Supermix (Bio‐Rad, USA).

    Article Title: Diarylheptanoids oxyphyllacinol, yakuchinone A, and yakuchinone B attenuate 12-O-tetradecanoylphorbol-13-acetate-induced acute skin inflammation in mice
    Article Snippet: Diarylheptanoids from Alpinia oxyphylla are potential anti-inflammatory compounds, but existing evidence is limited and has largely focused on YAK-A and YAK-B, with little direct comparative evaluation of structurally related diarylheptanoids in inflammatory models.. Here, we compared the protective effects of oxyphyllacinol (OXY), yakuchinone A (YAK-A), and yakuchinone B (YAK-B) in a 12-O-tetradecanoylphorbol-13-acetate (TPA)induced mouse ear inflammation model. Topical pretreatment with OXY, YAK-A, or YAK-B significantly attenuated TPA-induced ear edema and tissue thickening, with YAK-B showing the greatest reduction in edema under the experimental conditions examined.. These compounds differentially regulated inflammatory cytokine expression, with OXY showing more consistent inhibition of IL-1β and IL-6 at the protein level.

    Article Title: Enhanced Prophylactic Nephroprotective Effects of Nanoliposomal Nootkatone Against Cisplatin-Induced Renal Injury: Associations with NRF2/HO-1, TLR4/NF-κB, Apoptosis-Related (Bax/Bcl-2/Caspase-3), and Ferroptosis-Related (GPX4/SLC7A11) Markers.
    Article Snippet: This study investigated the prophylactic nephroprotective effects of crude nootkatone (NKT) and its nanoliposomal formulation (NKT-LNPs) against cisplatin-induced renal injury in rats.. Sixty male Sprague–Dawley rats were randomly allocated into six groups: control, NKT, NKT-LNPs, cisplatin, cisplatin + NKT, and cisplatin + NKT-LNPs.. NKT or NKT-LNPs (10 mg/kg NKT equivalent) was administered orally as prophylactic treatment for 7 days, with a single cisplatin injection (7.5 mg/kg, i.p.) on day 7.

    Article Title: Foundational characterization of tomato fruit RALF peptides reveals structural and functional specialization within the SlRALF family.
    Article Snippet: RNA integrity was verified by agarose gel electrophoresis, and RNA purity was assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific, USA). .. For cDNA synthesis, 1 μg of total RNA was used for cDNA synthesis using oligo(dT) primers and the iScript cDNA Synthesis Kit (Bio-Rad) following the manufactureŕs instructions. .. RT-qPCR was performed using SsoFast EvaGreen Supermix (Bio-Rad) on a CFX384 Real-Time PCR Detection System (Bio-Rad).

    Article Title: Modelling herpes simplex virus type 2 and Zika virus replication in mouse vaginal organoids and human vaginal spheroids.
    Article Snippet: Organoid pellets were resuspended in 300 μL of RNA lysis buffer (RNeasy Mini Kit, Qiagen Cat. No. 74104), and RNA was extracted according to the manufacturer’s instructions. .. 1 μg of RNA was reverse transcribed to cDNA using the iScript cDNA Synthesis Kit (BioRad Cat No. 1708891). .. RT-qPCR was performed using QuantStudio 3 (Thermo Fisher) and PowerTrack SYBR Green Master Mix (Thermo Fisher Cat No. A46110).

    Article Title: Arginine Supplementation for Juvenile Florida Pompano ( Trachinotus carolinus ) in Practical Plant‐Based Diets
    Article Snippet: .. Complementary DNA was synthesized using the iScript cDNA Synthesis Kit (Bio‐Rad Laboratories, Hercules, California, USA). ..

    Control:

    Article Title: A Combination of Ursolic Acid and Oleanolic Acid Disrupts Bioenergetic Homeostasis in Prostate Cancer and Inhibits Cancer Growth by Cytotoxic Autophagy.
    Article Snippet: .. Total RNA from treated and control cells was isolated using Aurum Total RNA Mini Kit (Bio-Rad Laboratories, Hercules, CA, USA) and cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad Laboratories) as per manufacturer protocol. .. The qPCR was performed on Applied Biosystems QuantStudio 3 Real-Time PCR system using the Universal SYBR Green Fast qPCR mix (Bio-Rad Laboratories) as described before [57].

    Isolation:

    Article Title: A Combination of Ursolic Acid and Oleanolic Acid Disrupts Bioenergetic Homeostasis in Prostate Cancer and Inhibits Cancer Growth by Cytotoxic Autophagy.
    Article Snippet: .. Total RNA from treated and control cells was isolated using Aurum Total RNA Mini Kit (Bio-Rad Laboratories, Hercules, CA, USA) and cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad Laboratories) as per manufacturer protocol. .. The qPCR was performed on Applied Biosystems QuantStudio 3 Real-Time PCR system using the Universal SYBR Green Fast qPCR mix (Bio-Rad Laboratories) as described before [57].

    Purification:

    Article Title: Enhanced Prophylactic Nephroprotective Effects of Nanoliposomal Nootkatone Against Cisplatin-Induced Renal Injury: Associations with NRF2/HO-1, TLR4/NF-κB, Apoptosis-Related (Bax/Bcl-2/Caspase-3), and Ferroptosis-Related (GPX4/SLC7A11) Markers.
    Article Snippet: This study investigated the prophylactic nephroprotective effects of crude nootkatone (NKT) and its nanoliposomal formulation (NKT-LNPs) against cisplatin-induced renal injury in rats.. Sixty male Sprague–Dawley rats were randomly allocated into six groups: control, NKT, NKT-LNPs, cisplatin, cisplatin + NKT, and cisplatin + NKT-LNPs.. NKT or NKT-LNPs (10 mg/kg NKT equivalent) was administered orally as prophylactic treatment for 7 days, with a single cisplatin injection (7.5 mg/kg, i.p.) on day 7.

    Reverse Transcription:

    Article Title: Modelling herpes simplex virus type 2 and Zika virus replication in mouse vaginal organoids and human vaginal spheroids.
    Article Snippet: Organoid pellets were resuspended in 300 μL of RNA lysis buffer (RNeasy Mini Kit, Qiagen Cat. No. 74104), and RNA was extracted according to the manufacturer’s instructions. .. 1 μg of RNA was reverse transcribed to cDNA using the iScript cDNA Synthesis Kit (BioRad Cat No. 1708891). .. RT-qPCR was performed using QuantStudio 3 (Thermo Fisher) and PowerTrack SYBR Green Master Mix (Thermo Fisher Cat No. A46110).



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